Figshare repository for "De novo transcriptome assembly and genome annotation of the fat-tailed dunnart (Sminthopsis crassicaudata)"

For additional info or help with contents, please contact Neke Ibeh: oibeh@student.unimelb.edu.au


CONTENTS

Dunnart_asm_12-2021_sm.fa
- The draft genome assembly of the fat-tailed dunnart. We employed a hybrid approach, which integrated ONT and PacBio long-read data with Illumina paired-end short reads.

Dunnart_asm_12-2021_sm_BUSCO_summary.txt
- BUSCO (v5.2.2) summary statistics for the draft genome assembly.

Dunnart_trinity_k29_output.Trinity.fasta
- Trinity (v2.13.2) de novo multi-tissue transcriptome assembly. We used a total of 24 dunnart RNA-seq samples, originating from the liver, testis, prostate, ovary, oviduct, uterus, eye, whole neonate, allantois, amnion, distal yolk sac, proximal yolk sac, and endometrium.

Dunnart_trinity_k29_BUSCO_summary.txt
- BUSCO (v5.2.2) summary statistics for the transcriptome assembly.

transcriptome_evaluation.txt
- Code and parameters used for filtering the transcriptome, evaluating transcriptome quality, ORF prediction, and transcriptome functional annotation.

maker_bopts.ctl
- MAKER control file specifying the parameters for the evidence aligners.

maker_opts.ctl
- MAKER control file specifying the parameters for the decision making process during gene building.

Sminthopsis_crassicaudata_1.gtf
- De novo annotation of the fat-tailed dunnart genome. Annotation of the dunnart genome was conducted using a combination of ab initio gene prediction algorithms and homology-based methods.